Technical Notes

ELISA

This protocol automates colorimetric protein detection from liver tissue extracts or plasma samples using enzyme-linked immunosorbent assay (ELISA) technology on the Transcriptic platform.

MSD Assay

This protocol automates colorimetric protein detection and quantitation from mouse tissue extracts or plasma samples using electroluminescence (ECL) technology on the Meso Scale Discovery (MSD) Sector S600 device.

RNA Isolation

This protocol automates RNA isolation from bacterial cultures and mouse liver tissue samples using magnetic bead-based technology on the Transcriptic platform.

Quantitative PCR

This protocol automates quantitative PCR (qPCR) for real time DNA detection and quantitation on Transcriptic’s robotic platform.

Protein Thermal Shift Assay

This protocol automates the thermal shift assay on the Transcriptic platform. By detecting changes in a protein’s thermal stability, this assay can be used for a variety of applications during drug development.